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71.
72.
R. Niloshini Sinnatamby Milton Shears J. Brian Dempson Michael Power 《Journal of thermal biology》2013
Predicted increases in temperature associated with climate change are expected to have consequences for fish, in particular for Arctic charr, Salvelinus alpinus, a cold-adapted fish species. Despite differences in predicted hydroecological responses to climate change in fluvial and lacustrine environments, little is known of whether fluvial and lacustrine Arctic charr populations may respond differently to increasing temperatures. In order to compare growth and thermal habitat use between habitat types, otolith-inferred average water temperatures estimated from whole otoliths and fork lengths at capture were measured for young-of-the-year (YOY) Arctic charr obtained from two proximal fluvial and lacustrine sites in Labrador, Canada. Otolith-inferred average experienced water temperatures were not significantly correlated with air temperatures at both sites, suggestive of behavioural thermoregulation by YOY. The majority of Kogluktokoluk Brook (fluvial) YOY were found using water temperatures consistent with laboratory determined preferred temperatures for juvenile Arctic charr, whereas most Tom's Pond (lacustrine) YOY were found using temperatures ranging between preferred temperatures and optimal temperatures for growth. There was no consistent difference between mean water temperatures used between YOY from the two sites. Otolith-inferred average experienced water temperatures were only correlated to fork lengths in Tom's Pond YOY. The lack of correlation in Kogluktokoluk Brook YOY is argued to reflect resource partitioning occurring as a result of territoriality known to occur among stream salmonids. The limited range of temperatures used by fluvial YOY in this study, particularly the lack of cooler temperatures, also suggests that fluvial YOY may face barriers to thermal refugia, and as a result may be particularly vulnerable to climate change. 相似文献
73.
Ana Nobre Nuno Empadinhas Maria Fernanda Nobre Eva Correia Lourenço Christopher Maycock Maria Rita Ventura Ana Mingote Milton S. da Costa 《Planta》2013,237(3):891-901
A mannosylglycerate synthase (MgS) gene detected in the genome of Selaginella moellendorffii was expressed in E. coli and the recombinant enzyme was purified and characterized. A remarkable and unprecedented feature of this enzyme was the ability to efficiently synthesize mannosylglycerate (MG) and glucosylglycerate (GG) alike, with maximal activity at 50 °C, pH 8.0 and with Mg2+ as reaction enhancer. We have also identified a novel glycoside hydrolase gene in this plant’s genome, which was functionally confirmed to be highly specific for the hydrolysis of MG and GG and named MG hydrolase (MgH), due to its homology with bacterial MgHs. The recombinant enzyme was maximally active at 40 °C and at pH 6.0–6.5. The activity was independent of cations, but Mn2+ was a strong stimulator. Regardless of these efficient enzymatic resources we could not detect MG or GG in S. moellendorffii or in the extracts of five additional Selaginella species. Herein, we describe the properties of the first eukaryotic enzymes for the synthesis and hydrolysis of the compatible solutes, MG and GG. 相似文献
74.
Peter Kivisto I.C. Jarvie J. Milton Yinger Marc V. Levine Robert Paine 《Ethnic and racial studies》2013,36(1):167-177
Andrew Hacker, TWO NATIONS: BLACK AND WHITE, SEPARATE, HOSTILE, UNEQUAL, New York: Charles Scribner's Sons, 1992, vii + 257 pp., $24.95. Catherine Silk and John Silk, RACISM AND ANTI‐RACISM IN AMERICAN POPULAR CULTURE, Manchester: Manchester University Press, 1990, x + 186 pp., £27.50. Therese Daniels and Jane Gerson (eds), THE COLOUR BLACK, BLACK IMAGES IN BRITISH TELEVISION, London: BFI Publishing, 1989, 160 pp., £4.95 (paper). Jim Pines and Paul Willemen (eds), QUESTIONS OF THIRD CINEMA, London: BFI Publishing, 1989, x + 246 pp., £7.95 (paper). David R. Roediger, THE WAGES OF WHITENESS: RACE AND THE MAKING OF THE AMERICAN WORKING CLASS, London: Verso, 1991, 191 pp., £12.95 (paper). William Cohen, AT FREEDOM'S EDGE: BLACK MOBILITY AND THE SOUTHERN WHITE QUEST FOR RACIAL CONTROL, 1861–1915, Baton Rouge: Louisiana State University Press, 1991, 340 pp., $42.50. Robert L. Cooper, LANGUAGE PLANNING AND SOCIAL CHANGE, Cambridge: Cambridge University Press, 1990, 216 pp., £27.50, £9.95 (paper). Emmanual Sivan and Menachem Friedman (eds), RELIGIOUS RADICALISM AND POLITICS IN THE MIDDLE EAST, Albany: State University of New York Press, 1990, 244 pp., n.p. Eliezer Ben‐Rafael and Stephen Sharot, ETHNICITY, RELIGION, AND CLASS IN ISRAELI SOCIETY, Cambridge: Cambridge University Press, 1991, 287 pp., £32.50. 相似文献
75.
Zufeng Ding Adam Milton Mizeracki Changping HuJawahar L. Mehta 《Biochemical and biophysical research communications》2013
Background
Atherosclerosis is associated with macrophage accumulation. LOX-1 has been shown to induce macrophage attachment, and its deletion (LOX-1 knockout, KO) reduces atherosclerosis in LDLr KO mice fed a high cholesterol diet. We examined differences in macrophage trafficking in age-matched wild type, LOX-1 KO, LDLr KO, and LDLr/LOX-1 double KO mice.Methods
Sections of aortas of mice fed high cholesterol diet were collected at weeks 0, 4, 8, 12 and 19 and analyzed by immunohistochemistry and flow cytometry.Results
In the LDLr KO mice aorta, CD68 positivity (macrophage accumulation) increased over time up to 12 weeks, and then the accumulation fell modestly but significantly. The periaortal fat and adventitia showed more CD68 positivity than the media and intima. This pattern was also evident in the non-atherosclerotic areas. Importantly, LOX-1 KO and LDLr–LOX-1 double KO mice showed diminished CD68 positivity in comparison to wild type and LDLR KO mice, respectively. Further, macrophages from LOX-1 KO mice revealed a marked reduction in migration (vs. macrophages from wild type mice) in in vitro migration assay.Conclusions
LOX-1 deletion translates into reduction in macrophage trafficking in the aorta of LDLr KO mice. Most of the macrophage trafficking appears in the subadventitial regions. 相似文献76.
77.
Maria Bromley Milton Bj?rn B?rsbo Graciela Rovner ?sa Lundgren-Nilsson Katharina Stibrant-Sunnerhagen Bj?rn Gerdle 《PloS one》2013,8(6)
Background
Incorporating the patient''s view on care and treatment has become increasingly important for health care. Patients describe the variety of consequences of their chronic pain conditions as significant pain intensity, depression, and anxiety. We hypothesised that intensities of common symptoms in chronic pain conditions carry important information that can be used to identify clinically relevant subgroups. This study has three aims: 1) to determine the importance of different symptoms with respect to participation and ill-health; 2) to identify subgroups based on data concerning important symptoms; and 3) to determine the secondary consequences for the identified subgroups with respect to participation and health factors.Methods and Subjects
This study is based on a cohort of patients referred to a multidisciplinary pain centre at a university hospital (n = 4645, participation rate 88%) in Sweden. The patients answered a number of questionnaires concerning symptoms, participation, and health aspects as a part of the Swedish Quality Registry for Pain Rehabilitation (SQRP).Results
Common symptoms (such as pain intensity, depression, and anxiety) in patients with chronic pain showed great variability across subjects and 60% of the cohort had normal values with respect to depressive and anxiety symptoms. Pain intensity more than psychological symptoms showed stronger relationships with participation and health. It was possible to identify subgroups based on pain intensity, depression, and anxiety. With respect to participation and health, high depressive symptomatology had greater negative consequences than high anxiety.Conclusions
Common symptoms (such as pain intensity and depressive and anxiety symptoms) in chronic pain conditions carry important information that can be used to identify clinically relevant subgroups. 相似文献78.
Anelena L. de Carvalho Bruce W. Nelson Milton C. Bianchini Daniela Plagnol Tatiana M. Kuplich Douglas C. Daly 《PloS one》2013,8(1)
We map the extent, infer the life-cycle length and describe spatial and temporal patterns of flowering of sarmentose bamboos (Guadua spp) in upland forests of the southwest Amazon. We first examine the spectra and the spectral separation of forests with different bamboo life stages. False-color composites from orbital sensors going back to 1975 are capable of distinguishing life stages. These woody bamboos flower produce massive quantities of seeds and then die. Life stage is synchronized, forming a single cohort within each population. Bamboo dominates at least 161,500 km2 of forest, coincident with an area of recent or ongoing tectonic uplift, rapid mechanical erosion and poorly drained soils rich in exchangeable cations. Each bamboo population is confined to a single spatially continuous patch or to a core patch with small outliers. Using spatial congruence between pairs of mature-stage maps from different years, we estimate an average life cycle of 27–28 y. It is now possible to predict exactly where and approximately when new bamboo mortality events will occur. We also map 74 bamboo populations that flowered between 2001 and 2008 over the entire domain of bamboo-dominated forest. Population size averaged 330 km2. Flowering events of these populations are temporally and/or spatially separated, restricting or preventing gene exchange. Nonetheless, adjacent populations flower closer in time than expected by chance, forming flowering waves. This may be a consequence of allochronic divergence from fewer ancestral populations and suggests a long history of widespread bamboo in the southwest Amazon. 相似文献
79.
Marcelo Magioli Katia Maria Paschoaletto Micchi de Barros Ferraz Eleonore Zulnara Freire Setz Alexandre Reis Percequillo Michelle Viviane de Sá Santos Rondon Vanessa Villanova Kuhnen Mariana Cristina da Silva Canhoto Karen Evelyn Almeida dos Santos Claudia Zukeran Kanda Gabriela de Lima Fregonezi Helena Alves do Prado Mitra Katherina Ferreira Milton Cezar Ribeiro Priscilla Marqui Schmidt Villela Luiz Lehmann Coutinho Márcia Gonçalves Rodrigues 《European Journal of Wildlife Research》2016,62(4):431-446
80.
Mariana Milano Milton Ozório Moraes Rodrigo Rodenbusch Caroline Xavier Carvalho Melaine Delcroix Gabriel Mousquer Lucas Laux da Costa Gisela Unis Elis Regina Dalla Costa Maria Lucia Rosa Rossetti 《PloS one》2016,11(2)
In Mycobacterium tuberculosis (MTB) infection, the complex interaction of host immune system and the mycobacteria is associated with levels of cytokines production that play a major role in determining the outcome of the disease. Several single-nucleotide polymorphisms (SNPs) in cytokine genes have been associated with tuberculosis (TB) outcome. The aim of this study was to evaluate the association between previously reported SNPs IL2–330 T>G (rs2069762); IL4–590 C>T (rs2243250); IL6–174 G>C (rs1800795); IL10–592 A>C (rs1800872); IL10–1082 G>A (rs1800896); IL17A -692 C>T (rs8193036); IL17A -197 G>A (rs2275913); TNF -238 G>A (rs361525); TNF -308 G>A (rs1800629) and IFNG +874 T>A (rs2430561) and pulmonary TB (PTB) susceptibility. We conducted a case-control study in individuals from Southern Brazil who were recruited between February 2012 and October 2013 in a high incidence TB city. We performed a multiplex genotyping assay in 191 patients with PTB and 175 healthy subjects. Our results suggest a decreased risk for PTB development associated with the IL17A -197A allele (OR = 0.29; p = 0.04), AA genotype (OR = 0.12; p = 0.04) and A carrier (AG/AA) (OR = 0.29; p = 0.004) and IL6 -174C carrier (CC/CG) (OR = 0.46; p = 0.04). We could not properly analyze IL17A -692 C>T (rs8193036) and IFNG +874T>A due to genotypic inconsistencies and found no evidence of association for the IL2, IL4, IL10 and TNF polymorphisms and PTB. In conclusion, our results show a protective effect of IL17 and IL6 polymorphisms on PTB outcome in Southern Brazilian population. 相似文献